The biosynthesis of radioactive fatty acids and cholesterol.

نویسندگان

  • R O BRADY
  • S GURIN
چکیده

The biological synthesis of long chain fatty acids from acetate by slices of rat liver tissue was demonstrated by Bloch, Borek, and Rittenberg (1) in 1946, and has been repeated by several investigators (2, 3). The effect of insulin on this incorporation was studied by Bloch and Kramer (2), who reported that, whereas insulin inhibited the incorporation of labeled acetate into fatty acids, the combination of insulin and non-labeled pyruvate resulted in an increased incorporation of the labeled acetate. It was of interest to determine whether other short chain fatty acids, as well as acetic acid, might be utilized by liver slices for the synthesis of higher fatty acids. This report isaccordingly concerned with the utilization of carboxyl and methyl-C4-acetic acid, /3-C14-pyruvic acid, and carboxylC4-butyric, hexanoic, and octanoic acids for this biosynthesis. The experiments reported here reveal some information concerning the mechanism by which these substrates are incorporated into fatty acids. A study of the effect of insulin upon this process has also been made.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 186 2  شماره 

صفحات  -

تاریخ انتشار 1950